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Basically, it is the surrounding water that is formed around proteins, also referred to as “protein hydration”, which allows homogeneous dispersion in solution and interactions by hydrogen bonds. We will first describe what the hydration shell of a protein is, because it plays a very important role in protein folding and function and is the target of all protein precipitation methods.
The main purpose of protein precipitation is to separate the protein from the solution either to eliminate interferences or to purify them.ĭepending on the solubility and molecular structure of the protein, the efficacy of various precipitation methods can be different.
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